Canada's Sister first appears in The Canadian Siblings. She has yet to make an appearance in the common comic style, or even in everyday clothing. Although a woman and sporting levels of body hair that rivals her brother, it is stated that it should not come off as a surprise. "Besides, how else did you think they could keep warm during those icy summers that everybody knows Canada has?"
Sister Canada's first appearance in a comic would be Yogurt As Far As The Eye Can See. She was shown in the first panel of the comic, talking to an unknown person on the phone, which happens to be her brother.